enzyme kinetics module sigmaplot, version 12.2 Search Results


cas9 enzyme  (Integrated DNA Technologies)
99
Integrated DNA Technologies cas9 enzyme
( A ) Top: ChIP-qPCR to measure RNAP occupancy: Schematic of hsp70 ( F44E5.4/.5 ) gene regions within the Promoter (−390 to −241), middle of gene (Region B: +696 to +915) and towards 3’-UTR (Region C: +1827 to +1996) assayed for occupancy by RNAP. Schematic of hsp70 ( C12C8.1 ) gene regions close to the beginning (Region A: +25 to +185), middle of gene (Region B: +475 to +583) and towards 3’-UTR (Region C:+1645 to +1835) assayed for occupancy by RNAP. Bottom: RNAP occupancy at these regions in control non-heat shocked wild-type and tph-1 mutant animals. RNAP (% input) at the Region A/Promoter region in control wild-type animals does not significantly differ from that at Region B or Region ( C ) as would have been expected if RNAP was paused near the TSS (n = 8 experiments). Data show Mean ± Standard Error of the Mean. Comparisons are non-significant. ANOVA with Tukey’s correction. ( B ) Top: Schematic of hsf-1 tagged at the endogenous locus with 3X FLAG using <t>CRISPR/Cas9.</t> Bottom: Representative micrographs of confocal sections of the germline region showing HSF-1 immunostaining with anti-FLAG antibody in wild-type and tph-1 mutants (n = 5 experiments). Note HSF-1 is expressed in germline cells in both wild-type animals and tph-1 mutant animals. Scale bar = 10 µm. ( C ) Fold change in HSF-1 occupancy at the promoter of a control gene not having any HSF-1 binding sites ( syp-1 ) in wild-type animals and tph-1 mutants following 5 and 15 min at 34°C. % input values were normalized to that in control wild-type animals (n = 14 experiments). ( D ) Representative western blot using anti-FLAG antibody showing the specificity of the FLAG antibody (lane 1: Ctrl are wild-type animals that do not express FLAG-tagged proteins), and HSF-1 levels in wild-type and tph-1 animals under control and heat shock conditions. Tubulin was used as a loading control. ( E ) Quantified HSF-1 levels in wild-type and tph-1 animals under control and heat shock conditions (n = 3 experiments). Protein levels are normalized to control wild-type animals. Note HSF-1 protein levels do not change. Data in A, C, E show Mean ± Standard Error of the Mean. ( A and C ), ANOVA with Tukey’s correction. ( E ) Paired Student’s t-test. ns, non-significant.
Cas9 Enzyme, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cas9 enzyme/product/Integrated DNA Technologies
Average 99 stars, based on 1 article reviews
cas9 enzyme - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier
la taq enzyme  (TaKaRa)
95
TaKaRa la taq enzyme
( A ) Top: ChIP-qPCR to measure RNAP occupancy: Schematic of hsp70 ( F44E5.4/.5 ) gene regions within the Promoter (−390 to −241), middle of gene (Region B: +696 to +915) and towards 3’-UTR (Region C: +1827 to +1996) assayed for occupancy by RNAP. Schematic of hsp70 ( C12C8.1 ) gene regions close to the beginning (Region A: +25 to +185), middle of gene (Region B: +475 to +583) and towards 3’-UTR (Region C:+1645 to +1835) assayed for occupancy by RNAP. Bottom: RNAP occupancy at these regions in control non-heat shocked wild-type and tph-1 mutant animals. RNAP (% input) at the Region A/Promoter region in control wild-type animals does not significantly differ from that at Region B or Region ( C ) as would have been expected if RNAP was paused near the TSS (n = 8 experiments). Data show Mean ± Standard Error of the Mean. Comparisons are non-significant. ANOVA with Tukey’s correction. ( B ) Top: Schematic of hsf-1 tagged at the endogenous locus with 3X FLAG using <t>CRISPR/Cas9.</t> Bottom: Representative micrographs of confocal sections of the germline region showing HSF-1 immunostaining with anti-FLAG antibody in wild-type and tph-1 mutants (n = 5 experiments). Note HSF-1 is expressed in germline cells in both wild-type animals and tph-1 mutant animals. Scale bar = 10 µm. ( C ) Fold change in HSF-1 occupancy at the promoter of a control gene not having any HSF-1 binding sites ( syp-1 ) in wild-type animals and tph-1 mutants following 5 and 15 min at 34°C. % input values were normalized to that in control wild-type animals (n = 14 experiments). ( D ) Representative western blot using anti-FLAG antibody showing the specificity of the FLAG antibody (lane 1: Ctrl are wild-type animals that do not express FLAG-tagged proteins), and HSF-1 levels in wild-type and tph-1 animals under control and heat shock conditions. Tubulin was used as a loading control. ( E ) Quantified HSF-1 levels in wild-type and tph-1 animals under control and heat shock conditions (n = 3 experiments). Protein levels are normalized to control wild-type animals. Note HSF-1 protein levels do not change. Data in A, C, E show Mean ± Standard Error of the Mean. ( A and C ), ANOVA with Tukey’s correction. ( E ) Paired Student’s t-test. ns, non-significant.
La Taq Enzyme, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/la taq enzyme/product/TaKaRa
Average 95 stars, based on 1 article reviews
la taq enzyme - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
enzyme kinetics module sigmaplot, version 12.2  (SPSS Inc)
90
SPSS Inc enzyme kinetics module sigmaplot, version 12.2
( A ) Top: ChIP-qPCR to measure RNAP occupancy: Schematic of hsp70 ( F44E5.4/.5 ) gene regions within the Promoter (−390 to −241), middle of gene (Region B: +696 to +915) and towards 3’-UTR (Region C: +1827 to +1996) assayed for occupancy by RNAP. Schematic of hsp70 ( C12C8.1 ) gene regions close to the beginning (Region A: +25 to +185), middle of gene (Region B: +475 to +583) and towards 3’-UTR (Region C:+1645 to +1835) assayed for occupancy by RNAP. Bottom: RNAP occupancy at these regions in control non-heat shocked wild-type and tph-1 mutant animals. RNAP (% input) at the Region A/Promoter region in control wild-type animals does not significantly differ from that at Region B or Region ( C ) as would have been expected if RNAP was paused near the TSS (n = 8 experiments). Data show Mean ± Standard Error of the Mean. Comparisons are non-significant. ANOVA with Tukey’s correction. ( B ) Top: Schematic of hsf-1 tagged at the endogenous locus with 3X FLAG using <t>CRISPR/Cas9.</t> Bottom: Representative micrographs of confocal sections of the germline region showing HSF-1 immunostaining with anti-FLAG antibody in wild-type and tph-1 mutants (n = 5 experiments). Note HSF-1 is expressed in germline cells in both wild-type animals and tph-1 mutant animals. Scale bar = 10 µm. ( C ) Fold change in HSF-1 occupancy at the promoter of a control gene not having any HSF-1 binding sites ( syp-1 ) in wild-type animals and tph-1 mutants following 5 and 15 min at 34°C. % input values were normalized to that in control wild-type animals (n = 14 experiments). ( D ) Representative western blot using anti-FLAG antibody showing the specificity of the FLAG antibody (lane 1: Ctrl are wild-type animals that do not express FLAG-tagged proteins), and HSF-1 levels in wild-type and tph-1 animals under control and heat shock conditions. Tubulin was used as a loading control. ( E ) Quantified HSF-1 levels in wild-type and tph-1 animals under control and heat shock conditions (n = 3 experiments). Protein levels are normalized to control wild-type animals. Note HSF-1 protein levels do not change. Data in A, C, E show Mean ± Standard Error of the Mean. ( A and C ), ANOVA with Tukey’s correction. ( E ) Paired Student’s t-test. ns, non-significant.
Enzyme Kinetics Module Sigmaplot, Version 12.2, supplied by SPSS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/enzyme kinetics module sigmaplot, version 12.2/product/SPSS Inc
Average 90 stars, based on 1 article reviews
enzyme kinetics module sigmaplot, version 12.2 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
inbios elisa  (InBios International)
90
InBios International inbios elisa
Accuracy of <t> Panbio </t> <t> ELISA </t> for dengue diagnosis for patients from Panel A.
Inbios Elisa, supplied by InBios International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inbios elisa/product/InBios International
Average 90 stars, based on 1 article reviews
inbios elisa - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
elisa  (Bethyl)
90
Bethyl elisa
Accuracy of <t> Panbio </t> <t> ELISA </t> for dengue diagnosis for patients from Panel A.
Elisa, supplied by Bethyl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/elisa/product/Bethyl
Average 90 stars, based on 1 article reviews
elisa - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
enzymes  (Carna Inc)
96
Carna Inc enzymes
Accuracy of <t> Panbio </t> <t> ELISA </t> for dengue diagnosis for patients from Panel A.
Enzymes, supplied by Carna Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/enzymes/product/Carna Inc
Average 96 stars, based on 1 article reviews
enzymes - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
enzyme link immunosorbent assay elisa kits  (Bethyl)
93
Bethyl enzyme link immunosorbent assay elisa kits
Accuracy of <t> Panbio </t> <t> ELISA </t> for dengue diagnosis for patients from Panel A.
Enzyme Link Immunosorbent Assay Elisa Kits, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/enzyme link immunosorbent assay elisa kits/product/Bethyl
Average 93 stars, based on 1 article reviews
enzyme link immunosorbent assay elisa kits - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
enzyme immunoassay 11-dhtxb2 eia kit  (Cayman Chemical)
90
Cayman Chemical enzyme immunoassay 11-dhtxb2 eia kit
Accuracy of <t> Panbio </t> <t> ELISA </t> for dengue diagnosis for patients from Panel A.
Enzyme Immunoassay 11 Dhtxb2 Eia Kit, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/enzyme immunoassay 11-dhtxb2 eia kit/product/Cayman Chemical
Average 90 stars, based on 1 article reviews
enzyme immunoassay 11-dhtxb2 eia kit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
lyme elisa test c6  (Immunetics)
90
Immunetics lyme elisa test c6
Accuracy of <t> Panbio </t> <t> ELISA </t> for dengue diagnosis for patients from Panel A.
Lyme Elisa Test C6, supplied by Immunetics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lyme elisa test c6/product/Immunetics
Average 90 stars, based on 1 article reviews
lyme elisa test c6 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
springer-verlag  (Verlag GmbH)
90
Verlag GmbH springer-verlag
Accuracy of <t> Panbio </t> <t> ELISA </t> for dengue diagnosis for patients from Panel A.
Springer Verlag, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/springer-verlag/product/Verlag GmbH
Average 90 stars, based on 1 article reviews
springer-verlag - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
elisa kits  (DEMEDITEC Diagnostics GmbH)
90
DEMEDITEC Diagnostics GmbH elisa kits
Accuracy of <t> Panbio </t> <t> ELISA </t> for dengue diagnosis for patients from Panel A.
Elisa Kits, supplied by DEMEDITEC Diagnostics GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/elisa kits/product/DEMEDITEC Diagnostics GmbH
Average 90 stars, based on 1 article reviews
elisa kits - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
itgα6 (122 pb) products  (Thermo Fisher)
90
Thermo Fisher itgα6 (122 pb) products
RFLP analysis in 3% agarose gel. ( A ) Enzymatic digestion of <t>ITGα6</t> (A380T) SNP with TaaI, lane 1: variant genotype (AA); lane 2: wild-type homozygous (GG) and lane 3: heterozygous (GA); ( B ) Enzymatic digestion of ITGβ4 (R1281W) mutation with BsrI, lanes 1–3: banding prototype for wild-type genotype (CC). PM: molecular weight of 50 bp.
Itgα6 (122 Pb) Products, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/itgα6 (122 pb) products/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
itgα6 (122 pb) products - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


( A ) Top: ChIP-qPCR to measure RNAP occupancy: Schematic of hsp70 ( F44E5.4/.5 ) gene regions within the Promoter (−390 to −241), middle of gene (Region B: +696 to +915) and towards 3’-UTR (Region C: +1827 to +1996) assayed for occupancy by RNAP. Schematic of hsp70 ( C12C8.1 ) gene regions close to the beginning (Region A: +25 to +185), middle of gene (Region B: +475 to +583) and towards 3’-UTR (Region C:+1645 to +1835) assayed for occupancy by RNAP. Bottom: RNAP occupancy at these regions in control non-heat shocked wild-type and tph-1 mutant animals. RNAP (% input) at the Region A/Promoter region in control wild-type animals does not significantly differ from that at Region B or Region ( C ) as would have been expected if RNAP was paused near the TSS (n = 8 experiments). Data show Mean ± Standard Error of the Mean. Comparisons are non-significant. ANOVA with Tukey’s correction. ( B ) Top: Schematic of hsf-1 tagged at the endogenous locus with 3X FLAG using CRISPR/Cas9. Bottom: Representative micrographs of confocal sections of the germline region showing HSF-1 immunostaining with anti-FLAG antibody in wild-type and tph-1 mutants (n = 5 experiments). Note HSF-1 is expressed in germline cells in both wild-type animals and tph-1 mutant animals. Scale bar = 10 µm. ( C ) Fold change in HSF-1 occupancy at the promoter of a control gene not having any HSF-1 binding sites ( syp-1 ) in wild-type animals and tph-1 mutants following 5 and 15 min at 34°C. % input values were normalized to that in control wild-type animals (n = 14 experiments). ( D ) Representative western blot using anti-FLAG antibody showing the specificity of the FLAG antibody (lane 1: Ctrl are wild-type animals that do not express FLAG-tagged proteins), and HSF-1 levels in wild-type and tph-1 animals under control and heat shock conditions. Tubulin was used as a loading control. ( E ) Quantified HSF-1 levels in wild-type and tph-1 animals under control and heat shock conditions (n = 3 experiments). Protein levels are normalized to control wild-type animals. Note HSF-1 protein levels do not change. Data in A, C, E show Mean ± Standard Error of the Mean. ( A and C ), ANOVA with Tukey’s correction. ( E ) Paired Student’s t-test. ns, non-significant.

Journal: eLife

Article Title: Serotonin signaling by maternal neurons upon stress ensures progeny survival

doi: 10.7554/eLife.55246

Figure Lengend Snippet: ( A ) Top: ChIP-qPCR to measure RNAP occupancy: Schematic of hsp70 ( F44E5.4/.5 ) gene regions within the Promoter (−390 to −241), middle of gene (Region B: +696 to +915) and towards 3’-UTR (Region C: +1827 to +1996) assayed for occupancy by RNAP. Schematic of hsp70 ( C12C8.1 ) gene regions close to the beginning (Region A: +25 to +185), middle of gene (Region B: +475 to +583) and towards 3’-UTR (Region C:+1645 to +1835) assayed for occupancy by RNAP. Bottom: RNAP occupancy at these regions in control non-heat shocked wild-type and tph-1 mutant animals. RNAP (% input) at the Region A/Promoter region in control wild-type animals does not significantly differ from that at Region B or Region ( C ) as would have been expected if RNAP was paused near the TSS (n = 8 experiments). Data show Mean ± Standard Error of the Mean. Comparisons are non-significant. ANOVA with Tukey’s correction. ( B ) Top: Schematic of hsf-1 tagged at the endogenous locus with 3X FLAG using CRISPR/Cas9. Bottom: Representative micrographs of confocal sections of the germline region showing HSF-1 immunostaining with anti-FLAG antibody in wild-type and tph-1 mutants (n = 5 experiments). Note HSF-1 is expressed in germline cells in both wild-type animals and tph-1 mutant animals. Scale bar = 10 µm. ( C ) Fold change in HSF-1 occupancy at the promoter of a control gene not having any HSF-1 binding sites ( syp-1 ) in wild-type animals and tph-1 mutants following 5 and 15 min at 34°C. % input values were normalized to that in control wild-type animals (n = 14 experiments). ( D ) Representative western blot using anti-FLAG antibody showing the specificity of the FLAG antibody (lane 1: Ctrl are wild-type animals that do not express FLAG-tagged proteins), and HSF-1 levels in wild-type and tph-1 animals under control and heat shock conditions. Tubulin was used as a loading control. ( E ) Quantified HSF-1 levels in wild-type and tph-1 animals under control and heat shock conditions (n = 3 experiments). Protein levels are normalized to control wild-type animals. Note HSF-1 protein levels do not change. Data in A, C, E show Mean ± Standard Error of the Mean. ( A and C ), ANOVA with Tukey’s correction. ( E ) Paired Student’s t-test. ns, non-significant.

Article Snippet: IDT Cas9 Enzyme , 12.2 µM.

Techniques: Mutagenesis, CRISPR, Immunostaining, Binding Assay, Western Blot

Journal: eLife

Article Title: Serotonin signaling by maternal neurons upon stress ensures progeny survival

doi: 10.7554/eLife.55246

Figure Lengend Snippet:

Article Snippet: IDT Cas9 Enzyme , 12.2 µM.

Techniques:

Accuracy of Panbio ELISA for dengue diagnosis for patients from Panel A.

Journal: Tropical Medicine and Infectious Disease

Article Title: Comparison of Two Commercial ELISA Kits for the Detection of Anti-Dengue IgM for Routine Dengue Diagnosis in Laos

doi: 10.3390/tropicalmed4030111

Figure Lengend Snippet: Accuracy of Panbio ELISA for dengue diagnosis for patients from Panel A.

Article Snippet: Of 74 admission samples, 16 (21.6%) were found positive by the Panbio ELISA, and 9 (12.2%) by the InBios ELISA.

Techniques: Enzyme-linked Immunosorbent Assay, Biomarker Discovery, Diagnostic Assay

Accuracy of InBios ELISA for dengue diagnosis for patients from Panel A.

Journal: Tropical Medicine and Infectious Disease

Article Title: Comparison of Two Commercial ELISA Kits for the Detection of Anti-Dengue IgM for Routine Dengue Diagnosis in Laos

doi: 10.3390/tropicalmed4030111

Figure Lengend Snippet: Accuracy of InBios ELISA for dengue diagnosis for patients from Panel A.

Article Snippet: Of 74 admission samples, 16 (21.6%) were found positive by the Panbio ELISA, and 9 (12.2%) by the InBios ELISA.

Techniques: Enzyme-linked Immunosorbent Assay, Biomarker Discovery, Diagnostic Assay

RFLP analysis in 3% agarose gel. ( A ) Enzymatic digestion of ITGα6 (A380T) SNP with TaaI, lane 1: variant genotype (AA); lane 2: wild-type homozygous (GG) and lane 3: heterozygous (GA); ( B ) Enzymatic digestion of ITGβ4 (R1281W) mutation with BsrI, lanes 1–3: banding prototype for wild-type genotype (CC). PM: molecular weight of 50 bp.

Journal: International Journal of Molecular Sciences

Article Title: α6β4 Integrin Genetic Variations (A380T and R1281W) and Breast Cancer Risk in an Argentinian Population

doi: 10.3390/ijms17101540

Figure Lengend Snippet: RFLP analysis in 3% agarose gel. ( A ) Enzymatic digestion of ITGα6 (A380T) SNP with TaaI, lane 1: variant genotype (AA); lane 2: wild-type homozygous (GG) and lane 3: heterozygous (GA); ( B ) Enzymatic digestion of ITGβ4 (R1281W) mutation with BsrI, lanes 1–3: banding prototype for wild-type genotype (CC). PM: molecular weight of 50 bp.

Article Snippet: For RFLP analysis, 5 µL of ITGα6 (122 pb) products were digested with TaaI restriction enzyme (Thermo Scientific) overnight at 65 °C.

Techniques: Agarose Gel Electrophoresis, Variant Assay, Mutagenesis, Molecular Weight

Sequencing results. ( A ) Genotype patterns for ITGα6 (A380T) loci, wild-type GG ( left ); heterozygous GA ( middle ) and variant genotype AA ( right ); ( B ) Wild-type pattern for ITGβ4 (R1281W) loci. Arrows indicate the variant position.

Journal: International Journal of Molecular Sciences

Article Title: α6β4 Integrin Genetic Variations (A380T and R1281W) and Breast Cancer Risk in an Argentinian Population

doi: 10.3390/ijms17101540

Figure Lengend Snippet: Sequencing results. ( A ) Genotype patterns for ITGα6 (A380T) loci, wild-type GG ( left ); heterozygous GA ( middle ) and variant genotype AA ( right ); ( B ) Wild-type pattern for ITGβ4 (R1281W) loci. Arrows indicate the variant position.

Article Snippet: For RFLP analysis, 5 µL of ITGα6 (122 pb) products were digested with TaaI restriction enzyme (Thermo Scientific) overnight at 65 °C.

Techniques: Sequencing, Variant Assay

Risk analysis of ITGα6 (A380T) SNP (G> A) based on inheritance models.

Journal: International Journal of Molecular Sciences

Article Title: α6β4 Integrin Genetic Variations (A380T and R1281W) and Breast Cancer Risk in an Argentinian Population

doi: 10.3390/ijms17101540

Figure Lengend Snippet: Risk analysis of ITGα6 (A380T) SNP (G> A) based on inheritance models.

Article Snippet: For RFLP analysis, 5 µL of ITGα6 (122 pb) products were digested with TaaI restriction enzyme (Thermo Scientific) overnight at 65 °C.

Techniques:

Allele distribution of ITGα6 (Ala380Thr) SNP in breast cancer patients and control subjects.

Journal: International Journal of Molecular Sciences

Article Title: α6β4 Integrin Genetic Variations (A380T and R1281W) and Breast Cancer Risk in an Argentinian Population

doi: 10.3390/ijms17101540

Figure Lengend Snippet: Allele distribution of ITGα6 (Ala380Thr) SNP in breast cancer patients and control subjects.

Article Snippet: For RFLP analysis, 5 µL of ITGα6 (122 pb) products were digested with TaaI restriction enzyme (Thermo Scientific) overnight at 65 °C.

Techniques: